Viruses 10: 147, (125) Sadana, P., Geyer, R., Pezoldt, J., Helmsing, S., Huehn, J., Hust, M., Dersch, P. and Scrima, A. Similar therapies already exist for other diseases such as diphtheria or tetanus. (79) Trott, M., Weiß, S., Antoni, S., Koch, J., von Briesen, H., Hust, M. and Dietrich, U. They enjoy working in the consortium, and it shows: “We are happy to be able to make a contribution to helping in the current situation,” says Nora Langreder, and Kai-Thomas Schneider adds: “This includes working on weekends or holidays. Norman Hack zusammen mit weiteren Instituten aus Braunschweig, München und Hannover Grundlagenforschung zum Thema „Additive Fertigung von Bauteilen“ betrieben. Mining gut microbiome oligopeptides by functional metaproteome display. Neutralization of Botulinum Neurotoxin Type E by a Humanized Antibody. (111) Froude, J.W., Pelat, T., Miethe, S., Zak, S.E., Wec, A.Z., Chandran, K., Brannan, J.M., Bakken, R.R., Hust, M., Thullier, P. and Dye, J.M. Bacterial flagellar capping proteins adopt diverse oligomeric states. (21) Jordan, E., Al-Halabi, L., Schirrmann, T., Hust, M. and Dübel, S. (2007). Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens. PMID: 31953428 PMCID: PMC6969050 DOI: 10.1038/s41598-019-57103-5 Abstract Diphtheria is an infectious disease caused by Corynebacterium diphtheriae. (139) Wenzel, E.V., Bosnak, M., Tierney, R., Schubert, M., Brown, J., Dübel, S., Efstratiou, A., Sesardic, D., Sticking, P. and Hust, M. (2020). Development of human-like scFv-Fc neutralizing botulinum neurotoxin E. (91) Avril, A., Miethe, S., Popoff, M., Mazuet, C., Chahboun, S., Rassetti-Escargueil, C., Sesardic, D., Thullier, P., Hust, M. and Pelat, T. (2015). Antibody Phage Display. (39) Schirrmann, T., Menzel, C., Hust, M., Prilop, J., Jostock, T. and Dübel, S. (2010). (145) Bertoglio, F., Meier, D., Langreder, N., Steinke, S., Rand, U., Simonelli, L., Heine, P. A., Ballmann, R., Schneider, K.-T., Roth, K. D. R., Ruschig, M., Riese, P., Eschke, K., Kim, Y., Schaeckermann, D., Pedotti, M., Kuhn, P., Zock-Emmenthal, S., Wöhrle, J., Becker, M., Grashoff, M., Wenzel, E. V., Russo, G., Kröger, A., Brunotte, L., Ludwig, S., Fühner, V., Krämer, S. D., Dübel, S., Varani, L., Roth, G., Cicin-Sain, L., Schubert, M., and Hust, M. (2020) SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface. Antibodies produced by this method have the advantage that they are not synthetic or chemically produced substances, but are the body’s own molecules and therefore have hardly any side effects. The invasin D protein from Yersinia pseudotuberculosis selectively binds the Fab region of host antibodies and affects colonization of the intestine. (34) Pelat, T., Hust, M., Hale, M., Lefranc, M.-P., Dübel, S. and Thullier, P. (2009). and Hust, M.. (121) Zantow, J., Moreira, G.M.S.G., Dübel, S. and Hust, M. (2018. (114) Stech, M., Nikolaeva, O., Thoring, L., Stöcklein, W.F.M., Wüstenhagen, D.A., Hust, M., Dübel, S. and Kubick, S. (2017). (126) Burke, C.W., Froude, J.W., Miethe, S., Hülseweh, B., Hust, M. and Glass, P.J. Rise and fall of an anti-MUC1 specific antibody. The binary toxin CDT of Clostridium difficile as tool for intracellular delivery of bacterial glucosyltransferase domains. Braunschweig TU Braunschweig ATAC (Antibody therapy against corona) Prof. Michael Hust TU Braunschweig, Karolinska Institut (Schweden), IRB (Schweiz), Joint Research Center Only after successful clinical trials can the drug reach hospitals and doctors’ practices. Antibodies play an important role in the fight against the corona virus: they prevent the virus from entering our cells. (2013). (61) Zhang, C., Helmsing, S., Zagrebelsky, M., Schirrmann, T., Marschall, A.L.J., Schüngel, M., Korte, M., Hust, M. and Dübel, S. (2012). (112) Schmitt, L.C., Rau, A., Seifert, O., Honer, J., Schmid, S., Hutt, M., Zantow, J., Hust, M., Dübel, S., Olayioye, M.A. (11) Voedisch, B., Menzel. Lecturer for molecular biotechnology. Generating recombinant antibodies to the complete human proteome. (62) Frenzel, A., Fröde, D., Meyer, T., Schirrmann, T. and Hust, M. (2012). and Aleixo, J.A.. (2016). Production of Antibody Fragments in the Gram-positive bacterium B. megaterium. (105) Miethe, S., Mazuet, C., Liu, Y., Tierney, R., Rasetti-Escargueil, C., Avril, A., Frenzel, A., Thullier, P., Pelat, T., Urbain, R., Fontayne, A., Sesardic, D., Hust, M. and Popoff, M.-R. (2016). Post-Exposure Protection in Mice against Sudan Virus by a Two Antibody Cocktail. (56) Lüders, S., David, F., Steinwand, M., Jordan, E., Hust, M., Dübel, S. and Franco-Lara, E. (2011). Recombinant antibody fragments allow repeated measurements of C-reactive protein with a quartz crystal microbalance immunosensor. Phage display, Ed: Lim, T.S. (52) Schirrmann, T., Meyer, T., Schütte, M., Frenzel, A., and Hust, M. (2011). Human antibodies targeting CD30+ lymphomas. Prof. Dr.-Ing. (130) Fühner, V., Heine, P.A., Helmsing, S., Goy, S., Heidepriem, J., Loeffler, F.F., Dübel, S., Gerhard, R. and Hust, M. (2018). C., Jordan, E., El-Ghezal, A., Schirrmann, T., Hust, M. und Jostock, T. (2005). (8) Kirsch, M., Zaman, M., Meier, D., Dübel, S. and Hust, M. (2005). Ausbildung und Beruf. In: Antibody engineering Vol 2, Ed: Kontermann, R. and Dübel, S.. (44) Breitling, F., Broders, O., Helmsing, S., Hust, M. and Dübel, S. (2010). (13) Hust, M., Meysing, M, Schirrmann, T., Selke, M., Meens, J., Gerlach, G.-F. and Dübel, S. (2006). Michael Hust studierte von 1993 bis 1999 Biologie an der Carl von Ossietzky Universität Oldenburg . Herzstiftung fördert Entwicklung neuer Antikörper-Therapie gegen Covid-19. (19) Pelat, T., Hust, M., Laffly. Spielmannstraße 7 Development of neutralizing scFv-Fc against botulinum neurotoxin A light chain from a macaque immune library. This way, people who are already ill could be cured and even risk patients or medical staff could be protected. (2016). Human Anti-Lipopolysaccharid (LPS) antibodies against Legionella with high species specificity. Vertical migration behaviour of diatom assemblages of Wadden Sea sediments (Dangast/Germany): A study using Cryo-scanning electron microscopy. whether the antibody is able to inhibit the corona virus from docking to the cells. Stefan Dübel/TU Braunschweig; HZI/Verena Meier; Hallbauer_Fioretti; Michael Hust/TU Braunschweig; Kai Mattern/Institut für Mikrotechnik/TU Braunschweig; PLRI; Foto: Falk-Martin Drescher / Braunschweig Stadtmarketing GmbH (47) Naseem, S., Meens, J., Jores, J., Heller, M., Dübel, S., Hust, M. and Gerlach, G.F. (2010). “Before Corona, each of us did our own research on his or her own project. Phage display vectors for the in vitro generation of human antibody fragments. Die TU Braunschweig (Prof. Michael Hust) ist Teil des COVID19-Konsortiums "ATAC" im Rahmen des EU Horizon2020 Corona-Förderprogramms. (49) Schirrmann, T. and Hust, M. (2010). : 0531 391-5760 Human-Like Neutralizing Antibodies Protect Mice from Aerosol Exposure with Western Equine Encephalitis Virus. “Human monoclonal antibodies, i.e. Prof. Dr. Michael Hust Technische Universität Braunschweig Institut für Biochemie, Biotechnologie und Bioinformatik Abteilung Biotechnologie Spielmannstraße 7 38106 Braunschweig Tel. Supernatant was harvested after 48 h. (128) Beer, L.A., Tatge, H., Schneider, C., Ruschig, M., Hust, M., Barton, J., Thiemann, S., Fühner, V., Russo, G. and Gerhard, R. (2018). Email: s.duebel@tu-braunschweig.de It's current labs are located in the Biocentre and the BRICS. Production of Single Chain Fragment Variable (scFv) Antibodies in Escherichia coli Using the LEX Bioreactor. (110) Frenzel, A., Schirmann, T., Dübel, S. and Hust, M. (2017). (20) Hust, M., Dübel, S. and Schirrmann, T. (2007). (141) Tundidor, Y., Ponce, L., Chao, L., Solozabal, J., Hust, M., Dübel, S. & Rojas, G. (2020). Structural differences of amyloid-β fibrils revealed by antibodies from phage display. Trends in Biotechnology 22, 8-14, (4) Meyer, T., Hust, M., Marquardt, J., Krumbein, W.E. antibodies from a single cell clone of human blood, are attached to the surface of a phage, a kind of ‘transport virus’. (51) Meyer, T.,  Stratmann-Selke, J., Meens, J., Schirrmann, T., Gerlach G.F., Frank, R., Dübel, S., Strutzberg-Minder, K. and Hust, M (2011). Michael Hust weiß, wovon er redet, wenn er über die Borreliose spricht. (92) Miethe, S., Rasetti-Escargueil, C., Avril, A., Liu, Y., Chahboun, S., Korkeala, H., Mazuet, C., Popoff, M.-R., Pelat, T., Thullier. Werden Sie Mitglied unserer akademischen Gemeinschaft - bewerben Sie sich an der TU Braunschweig! Affinity maturation by phage display. Bildnachweis: Michael Hust/TU Braunschweig. Affinity-matured variants derived from nimotuzumab keep the original fine specificity and exhibit superior biological activity. (101) Zantow, J., Dübel, S. and Hust, M. (2016). (113) Kuhn, P., Thiem, S., Steinert, M., Purvis, D., Lugmayr, V., Treutlein, U., Plobner, L. , Leiser, R.-M., Hust, M. and Dübel, S. (2017). Innovationspreis Niedersachsen. Perspectives for systematic in vitro antibody generation. (64) Meyer, T.,  Schirrmann, T., Frenzel, A., Miethe, S., Stratmann-Selke, J., Gerlach, G.F., Strutzberg-Minder, K., Dübel, S., and Hust, M. (2012). Expression of recombinant Antibodies. In addition, the team has also created such libraries from the blood of cured corona patients because the probability of finding a suitable antibody is even greater in such cases. ➜ Research Centers: The research profile of the TU Braunschweig is characterized by several interdisciplinary and cross-faculty research networks, which are organized as centers. (68) Bertz, A., Wöhl-Bruhn, S., Miethe, S., Tiersch, B., Koetz, J., Hust, M., Bunjes, H. and Menzel, H. (2013). Parameters affecting the display of antibodies on phage. In Braunschweig, so Hust, kann dies nur das Helmholtz-Zentrum für Infektionsforschung, einer seiner Partner. ➜ Research projects: The Collaborative Research Centres, Research Groups and Priority Programmes funded by the German Research Foundation (DFG) play a special role. In: Antibody engineering Vol 1, Ed: Kontermann, R. and Dübel, S.. (43) Hust, M. and Mersmann, M. (2010). A Multinational Consortium for the Development of Therapeutic Antibodies to Tier 1 Bioterrorism Agents: Protective scFv-Fc Antibody against Marburg Virus derived from Macaque Immune Library. Sprachen und interkulturelle Kommunikation, Graduiertenschulen und Graduiertenkollegs, Fakultät Architektur, Bauingenieurwesen und Umweltwissenschaften, Fakultät für Elektrotechnik, Informationstechnik, Physik, Fakultät für Geistes- und Erziehungswissenschaften, Institut für Biochemie, Biotechnologie und Bioinformatik, Institut für Chemie und Biologie des Meeres, Institut für Biochemie und Biotechnologie, Abteilung Biotechnologie, Technische Universität Braunschweig, Advances in Experimental Medicine and Biology. (115) Rasetti-Escargueil , C., Avril, A., Miethe, S., Mazuet, C., Derman, Y., Selby, K., Thullier, P., Pelat, T., Urbain, R., Fontayne, A., Korkeala, H., Sesardic, D., Hust, M. and Popoff, M.R. (100) Schieferdecker, A., Oberle, A., Thiele, B., Hofmann, F., Göthel, M, Miethe, S., Hust, M., Braig, F., Voigt, M., Koch-Nolte, F., Haag, F., Alawi, M., Indenbirken, D., Grundhoff, A., Bokemeyer, C., Bacher, U., Kröger, N. and Binder, M. (2016). Cetuximab (Erbitux). International Microbiology 2, 103-110, Stellen der TU Braunschweig Jobbörse des Career Service Merchandising Sponsoring- & Spendenleistungen Drittmittelgeförderte Forschungsprojekte, Semestertermine Lehrveranstaltungen Studiengänge von A-Z Informationen für Erstsemester TUCard, Status GITZ-Dienste Handbuch für TYPO3 (Intern) Corporate Design-Toolbox (Intern) Glossar (DE-EN) Meine Daten ändern Hochschulöffentliche Bekanntmachungen, Technische Universität Braunschweig Universitätsplatz 2 38106 Braunschweig Postfach: 38092 Braunschweig Telefon: +49 (0) 531 391-0. Wirkstoff-Kandidat von Forschern der TU Braunschweig geht in klinische Phase. Antibody Phage Display. “Finding such neutralizing antibody candidates was our most important milestone on the way to a passive immunotherapy against Covid-19, but there are still many more steps and tests to be done before the final drug is ready for use,” says Michael Hust. In order to find suitable antibody candidates against the corona virus, the team at Carolo-Wilhelmina is working at full speed. (2016). B., Hust, M. and Zielonka S. (in press). (38) Pelat, T., Hust., M. and Thullier, P. (2009). Development of recombinant antibodies for biodefense using phage display. (27) Kügler, J., Nieswandt, S., Gerlach, G.F., Meens, J., Schirrmann, T. and Hust, M. (2008). Kai-Thomas Schneider adds: “We have all learned a lot and have grown together as a team. BDA Braunschweig geht zurück an die Uni - Am Institut für Tragwerksentwurf (ITE) der TU Braunschweig wird unter der Leitung von Univ. In: Handbook of Therapeutic Monoclonal Antibodies: From Bench to Clinic, Ed: An, Z. and Strohl, W.. (32) Jordan, E., Al-Halabi, L., Schirrmann, T. and Hust, M. (2009). : 0531 391-5760 E-Mail: m.hust@tu-braunschweig.de Cell-free synthesis of functional antibodies using a coupled in vitro transcription-translation system based on CHO cell lysates. 38106 Braunschweig (87) Kügler, J., Wilke, S. Meier, D., Tomszak, F.,  Frenzel, A. , Schirrmann, T., Dübel, S.,  Garritsen, H.,  Hock, B., Toleikis, L., Schütte, M. and Hust, M. (2015). Harald Kloft und Juniorprofessor Dr. (sc. and Hust, M.. (116) Frenzel, A., Kügler, J., Helmsing, S., Meier, D., Schirrmann, T., Hust, M. and Dübel, S. (2017). Single chain (scFab) fragment. A., Schirrmann, T., Dübel, S. and Hust, M. (2013). The research of the TU scientists in the consortium contributes to the research focus “Infection and Therapeutics” at the TU Braunschweig. 12/2014 Appointed as professor for biotechnology (außerplanmäßiger Professor). Research group leader Room: 106 Phone: +49.531.391.5760 Fax: +49.531.391.5763 e-mail: m.hust@tu-bs.de. Krumbein and PD Dr. Erhard Rhiel. E.,  Condemine, F., Bottex, C., Vidal, D., Lefranc, M.P., Dübel, S. and Thullier, P. (2007). (142) Dübel, S., Hust, M., Frenzel, A. Regulatory T cells engineered with a novel insulin-specific chimeric antigen receptor as a candidate immunotherapy for type 1 diabetes. (54) Dammeyer, T., Steinwand, M., Krüger, S.C., Dübel, S., Hust, M. and Timmis, K.N. Dübel, S. and Reichert, J.M.. (82) Stech, M., Hust, M., Schulze, C., Dübel, S. and Kubick, S. (2014). (24) Hust, M., Toleikis, L. and Dübel, S. (2007). In: Antibody engineering, Ed: Kontermann, R. and Dübel, S.. (40) Mersmann, M., Meier, D., Mersmann, J., Helmsing, S., Nilsson, P., Gräslund S.,  Structural Genomics Consortium, Colwill, K., Hust, M. and Dübel, S. (2010). In: Antibody engineering Vol 1, Ed: Kontermann, R. and Dübel, S.. (42) Pelat, T., Hust, M. and Thullier, P. (2010). Production systems for recombinant antibodies. (18) Schirrmann, T., Hust, M., Konthur, Z., Frank, R., Toleikis, L. and Dübel, S. (2007). (2018). Selection of recombinant antibodies from antibody gene libraries. mycoides small colony type. Auszeichnungen für Corona-Antikörperforschung an der TU Braunschweig. Identification of immunogenic polypeptides from a Mycoplasma hyopneumoniae genome library by phage display. "Wir im Norden ‚können‘ weltweit ausstrahlende Biotech-Hochtechnologie - das möchten wir auch mit dem Namen ausdrücken", kommentierte Mitgründer Professor Michael Hust von der TU Braunschweig. Das Corat wurde von Stefan Dübel und Michael Hust (TU Braunschweig) zusammen mit Gundram Jung (Universität Tübingen) initiiert und ist mittlerweile auf ein Konsortium von 40 Wissenschaftlern und Ärzten aus akademischen Institutionen und der Industrie … (2016). They are part of the CORAT consortium that is developing a passive immunotherapy against the corona virus. (2017) The European AntibotABE Framework Program and Its Update: Development of Innovative Botulinum Antibodies. (17) Hust, M., Jostock, T., Menzel, C., Voedisch, B., Mohr, A., Brenneis, M., Kirsch, M.I., Meier, D. and Dübel, S. (2007). Erfahren Sie mehr über die Kontakte von Wolfgang Niemeier und über Jobs bei ähnlichen Unternehmen. TU Braunschweig is involved in the “PhoenixD” cluster at Leibniz Universität Hannover (LUH), which is researching new optical technologies. Targeting Aspergillus fumigatus Crf Transglycosylases With Neutralizing Antibody Is Relevant but Not Sufficient to Erase Fungal Burden in a Neutropenic Rat Model. Isolation of nanomolar scFvs of non-human primate origin, cross-neutralizing botulinum neurotoxins A1 and A2 by targeting their heavy chain. (AA), Dipl.-Ing. Expression rekombinanter Proteinpharmazeutika. That is our motivation.”. Ihr Ziel ist es, einen universellen Impfstoff gegen von Zecken übertragene Krankheiten zu entwickeln. A transplant immunome deep sequencing and screening platform defines a unique targetable epitope fingerprint of multiple myeloma. Kai-Thomas Schneider and Nora Langreder are PhD students at the Institute for Biochemistry, Biotechnology and Bioinformatics at the Technische Universität Braunschweig. Recombinant VHHA6 cDNA was cloned into the pCMX expression vector (contributed by M. Hust, TU Braunschweig), which encodes an optimized human IgG1 Fc domain. (108) Postel, S., Deredge, D., Bonsor, D.A., Yu, X., Diederichs, K., Helmsing, S., Vromen, A., Friedler, A., Hust, M., Egelman, E.H., Beckett, D., Wintrode, P.L. Antibody Engineering: Methods and Protocols, Ed: Chames, P.. (65) Wezler, X., Hust, M., Helmsing, S., Schirrmann, T. and Dübel, S. (2012). Isolation of a nanomolar scFv inhibiting the endopeptidase activity of botulinum toxin A, by single-round panning of an immune phage-displayed library of macaque origin. barton.jessica@mh-hannover.de. Diese Probe aufs Exempel, müssen dann Andere machen, denn Husts Labor an der TU fehlt die nötige Sicherheitsstufe, um mit dem hochinfektiösen Virus „SARS-CoV-2“ zu experimentieren. To support the CORAT consortium they have temporarily put their own projects on hold. Pyruvate dehydrogenase complex - enzyme 2, a new target for. Mating antibody phage display with proteomics. Using the antibody gene libraries, the team is now testing which antibodies bind to the protein envelope of the corona virus and thus prevent it from intruding into other cells. A human scFv antibody generation pipeline for proteome research. Isolation of scFv fragments specific to OmpD of Salmonella Typhimurium. The two have been pursuing their doctorate at the Institute for Biochemistry, Biotechnology and Bioinformatics for about a year. (14) Michalzik, M., Balck, A., Büttgenbach, S., Al-Halabi, L., Hust, M. and Dübel, S. (2006). and von Nickisch-Rosenegk, M. (2016). High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells. (10) Hust, M., Toleikis, L. and Dübel, S. (2005). Phage display, Ed: Lim, T.S. ➜ Clusters of Excellence: In the Excellence Strategy of the Federal Government and States in 2018, TU Braunschweig was successful with two applications. Dr. Hust specializes in building antibody gene libraries and … (127) Froude, J.W., Herbert, A.S., Pelat, T., Miethe, S., Zak, S.E., Brannan, J.M., Bakken, R.R., Steiner, A.R., Yin, G., Hallam, T.J., Sato, A.K., Hust, M., Thullier, P. and Dye, J.M. Restriction-Free Construction of a Phage-Presented Very Short Macrocyclic Peptide Library. (33) Hust, M., Thie, H., Schirrmann, T. and Dübel, S. (2009). Email: m.hust@tu-braunschweig.de More than 30 scientists and doctors from academic institutions and industry are now involved in the consortium. International Microbiology 6, 33-39, (3) Hust, M., Maiss, E., Jacobsen, H.-J. Identification of an epitope in the membrane proximal external region of gp41 targeted by neutralizing antibodies in plasma from an elite controller using an Env-tailored phage display library. Wissenschaftler aus Braunschweig haben am 11. (2021). Fructose 1,6-Bisphosphate Aldolase, a Novel Immunogenic Surface Protein on Listeria Species. (148) König, J, Hust, M. and Van den Heuvel, J. They were obtained from human blood in pre-Corona times and, put simply, contain the information for the basic construction of practically all antibodies that the human species can produce,” explains Kai-Thomas Schneider. 4/1998 - 7/1999 Diploma thesis: "Molekularbiologische Untersuchungen zur Diversität von Diatomeenarten und Nachweis der Expressionsraten von Diatomeen fcp Genen im Wattsediment" (Molecularbiological analysis of diatom diversity and investigations on expression rates of the diatom fcp genes in the Wadden Sea sediments) in the AG Geomikrobiologie of the Institut für Chemie und Biologie des Meeres under the supervison of Prof. W.E. (2014). Designing human antibodies by phage display. “At the institute we have numerous of these antibody gene libraries. Dübel, S. and Reichert, J.M.. (83) Wilke, S. and Hust, M. (2014). Epitope Mapping by Phage Display. Normally, the corresponding approval procedure takes about one and a half years or more. This is how we make a preliminary selection,” says Nora Langreder. (53) Thie, H., Toleikis, L., Li, J., von Wasielewski, R., Bastert, G., Schirrmann, T., Esteves, I. T., Behrens, C. K.,  Fournes, B., Fournier, N., de Romeuf, C., Hust, M. and Dübel, S. (2011). Influence of the hydromechanical stress and temperature on growth and antibody fragment production with Bacillus megaterium. Technische Universität Braunschweig and Kontermann, R.E. (71) Jäger, V.,  Büssow, K., Wagner, A., Weber, S., Hust, M., Frenzel, A. and Schirrmann, T. (2013). Phage display, Ed: Lim, T.S. and Dübel, S. (2018). Phage display, Ed: Lim, T.S. Funktionales Proteom-Display zur Identifikation von Biomarkern. Development of human and macaque antibodies using antibody phage display for the detection of equine encephalitis viruses. (103) Frenzel, A., Schirrmann, T. and Hust, M. (2016). (69) Miethe, S., Meyer, T., Wöhl-Bruhn, S.,  Frenzel. (2014). (66) Hust, M., Frenzel, A., Meyer, T., Schirrmann, T. and Dübel, S. (2012).