9/1999 - 7/2002 PhD thesis: "Rekombinante Antikörper gegen die NIa-Protease des Plum Pox Virus" (Recombinant antibodies against the NIa protease of the plum pox virus) at the Lehrgebiet Molekulargenetik (now Institut für Pflanzengenetik, Abteilung Pflanzenbiotechnologie), Universität Hannover (now Leibniz Universität Hannover) under the supervision of Prof. Dr. H.-J. Harald Kloft und Juniorprofessor Dr. (sc. Oligopeptide M13 Phage Display in Pathogen Research. 47 of the best inhibiting antibodies were handed over to the HZI for further testing. (68) Bertz, A., Wöhl-Bruhn, S., Miethe, S., Tiersch, B., Koetz, J., Hust, M., Bunjes, H. and Menzel, H. (2013). Phage display, Ed: Lim, T.S. : 0531 391-5760 (2017). PMID: 31953428 PMCID: PMC6969050 DOI: 10.1038/s41598-019-57103-5 Abstract Diphtheria is an infectious disease caused by Corynebacterium diphtheriae. - identification of disease related biomarkers (immunogenic oligopeptides) using phage display, - develoment of an anti-tick vaccine (GO-Bio8 project) (Info Video). (53) Thie, H., Toleikis, L., Li, J., von Wasielewski, R., Bastert, G., Schirrmann, T., Esteves, I. T., Behrens, C. K.,  Fournes, B., Fournier, N., de Romeuf, C., Hust, M. and Dübel, S. (2011). In: Handbook of Therapeutic Monoclonal Antibodies: From Bench to Clinic, Ed: An, Z. and Strohl, W.. (32) Jordan, E., Al-Halabi, L., Schirrmann, T. and Hust, M. (2009). Validation of the Production of Antibodies in Different Formats in the HEK 293 Transient Gene Expression System. Prof. Dr. Michael Hust Technische Universität Braunschweig Institut für Biochemie, Biotechnologie und Bioinformatik Abteilung Biotechnologie Spielmannstraße 7 38106 Braunschweig Tel. AntiBotABE, CDiff, DATMAB, HANTOX, ATAC (Antibody Therapy against Corona) and CORAT. 18.500 Studierende 1745 seit 3.700 Beschäftigte They enjoy working in the consortium, and it shows: “We are happy to be able to make a contribution to helping in the current situation,” says Nora Langreder, and Kai-Thomas Schneider adds: “This includes working on weekends or holidays. Production systems for recombinant antibodies. In Braunschweig, so Hust, kann dies nur das Helmholtz-Zentrum für Infektionsforschung, einer seiner Partner. (62) Frenzel, A., Fröde, D., Meyer, T., Schirrmann, T. and Hust, M. (2012). : 0531 391-5760 E-Mail: m.hust@tu-braunschweig.de Regulatory T cells engineered with a novel insulin-specific chimeric antigen receptor as a candidate immunotherapy for type 1 diabetes. (110) Frenzel, A., Schirmann, T., Dübel, S. and Hust, M. (2017). There, SARS-CoV-2 viruses are used to test whether the antibodies can block these viruses. Research interests Antibody Engineering: - antibodies for diagnostic and therapy with a focus on pathogens on toxins e.g. (85) Miethe, S., Pelat, T., Avril, A., Popoff, M., Molgo, J., Rasetti‑Escargueil, C., Fontayne, A., Korkeala, H., Thullier, P., Sesardic, D. and Hust, M. (2014). (88) Becker, M., Felsberger, A., Frenzel, A., Shattuck, W.M.C, Dyer, M., Kügler, J., Zantow, J., Mather, T. and Hust, M. (2015). They are obtained from immune cells in human blood and stored in small test tubes that can hold about 1.5 millilitres. Isolation and characterisation of a human-like antibody fragment (scFv) that inactivates VEEV in vitro and in vivo. Trendbericht Biochemie 2018: Menschliche Antikörper für Medikamente. (124) Russo, G., Theisen, U., Fahr, W., Helmsing, S., Hust, M., Köster R.W. (129) Chung, S.Y., Schöttelndreier, D., Tatge, H., Fühner, V., Hust, M., Beer, L.A. and Gerhard, R. (2018). (35) Schütte, M., Thullier, P., Pelat, T., Wezler, X., Rosenstock, P., Hinz, D., Kirsch, M.I., Hasenberg, M., Frank, R., Schirrmann, T., Gunzer, M., Hust, M. and Dübel, S. (2009). Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens. Dr. Hust specializes in building antibody gene libraries and … Selection of recombinant antibodies from antibody gene libraries. A., Schirrmann, T., Dübel, S. and Hust, M. (2013). (1999). Technische Universität Braunschweig Universitätsplatz 2 38106 Braunschweig Postfach: 38092 Braunschweig Telefon: +49 (0) 531 391-0. In: Antibody engineering Vol 1, Ed: Kontermann, R. and Dübel, S.. (41) Hust, M. and Dübel, S. (2010). Journal of Virological Methods 106, 225-233, (2) Hust, M., Krumbein, W.E. Email: m.hust@tu-braunschweig.de In: Antibody engineering Vol 2, Ed: Kontermann, R. and Dübel, S.. (44) Breitling, F., Broders, O., Helmsing, S., Hust, M. and Dübel, S. (2010). Even supposedly minor tasks such as ordering materials or sterilizing work equipment are important for research to function. (63) Rülker, T., Voß, L., Thullier, P., O'Brien, L.M., Pelat, T., Perkins, S.D., Langemann, C., Schirrmann, T., Dübel, S., Marschall, H.-J., Hust, M. and Hülseweh, B. (109) Noyan, F., Zimmermann, K., Hardtke-Wolenski, M., Knoefel, A., Schulde, E., Geffers, R., Hust, M., Huehn, J., Galla, M., Morgan, M., Jokuszies, A., Manns, M.P., Jaeckel, E. (2017). (50) Bourbeillon, J., Orchard, S., Benhar, I., Borrebaeck, C., de Daruvar, A., Dübel, S., Frank, R., Gibson, F., Gloriam, D., Haslam, N., Hiltker, T., Humphrey-Smith, I., Hust, M., Juncker, D., Koegl, M., Konthur, Z., Korn, B., Krobitsch, S., Muyldermans, S., Nygren, P.-A., Palcy, S., Polic, B., Rodriguez, H., Sawyer, A., Schlapshy, M., Snyder, M.,Stoevesandt, O., Taussig, M.J., Templin, M., Uhlen, M., van der Maarel, S., Wingren, C., Hermjakob, H. and Sherman, D. (2010). An der TU Braunschweig können solche Antikörper gentechnologisch komplett im Reagenzglas gewonnen werden. Pyruvate dehydrogenase complex - enzyme 2, a new target for. BDA Braunschweig geht zurück an die Uni - Am Institut für Tragwerksentwurf (ITE) der TU Braunschweig wird unter der Leitung von Univ. Institute of Biochemistry, Biotechnology and Bioinformatics 12/2014 Appointed as professor for biotechnology (außerplanmäßiger Professor). The invasin D protein from Yersinia pseudotuberculosis selectively binds the Fab region of host antibodies and affects colonization of the intestine. www.bbt.tu-bs.de/Biotech, Scientists and industrial partners in the Corona Antibody Team (CORAT consortium) are researching a passive immunisation therapy against the corona virus. Selection Strategies II: Antibody phage display. (6)Institute of Toxicology, Hannover Medical School, 30625 Hannover, Germany. Development of human and macaque antibodies using antibody phage display for the detection of equine encephalitis viruses. Similar therapies already exist for other diseases such as diphtheria or tetanus. In: Antibody engineering Vol 1, Ed: Kontermann, R. and Dübel, S.. (43) Hust, M. and Mersmann, M. (2010). Immune libraries from non-human primates (NHP). Generating recombinant antibodies for research, diagnostics and therapy using phage display. Antibody production by the Gram-positive bacterium Bacillus megaterium. Dübel, S. and Reichert, J.M.. (83) Wilke, S. and Hust, M. (2014). (77) Hust, M., Frenzel, A., Schirrmann, T. and Dübel, S. (2014). Linear discriminant analysis identifies mitochondrially localized proteins in Neurospora crassa. Antibody Phage Display. Development of human-like scFv-Fc neutralizing botulinum neurotoxin E. (91) Avril, A., Miethe, S., Popoff, M., Mazuet, C., Chahboun, S., Rassetti-Escargueil, C., Sesardic, D., Thullier, P., Hust, M. and Pelat, T. (2015). Wirkstoff-Kandidat von Forschern der TU Braunschweig geht in klinische Phase. Curriculum vitae 10/1993 - 7/1999 Studies of Biology at the Carl-von-Ossietzky Universität, Oldenburg. Antibody Phage Display. The work in the laboratory only works because everyone helps. In: Handbook of therapeutic antibodies, 2nd ed., Ed. (20) Hust, M., Dübel, S. and Schirrmann, T. (2007). Dem Braunschweiger ... sowie ein Team von TU-Biotechnologen um Prof. Michael Hust haben rund 6000 verschiedene künstlich hergestellte menschlichen Antikörper analysiert. A roadmap to generate renewable protein binders to the human proteome. Influence of the hydromechanical stress and temperature on growth and antibody fragment production with Bacillus megaterium. Suppression of p75 Neurotrophin Receptor Surface Expression with Intrabodies Influences Bcl-xL mRNA Expression and Neurite Outgrowth in PC12 Cells. TU Braunschweig is involved in the “PhoenixD” cluster at Leibniz Universität Hannover (LUH), which is researching new optical technologies. (61) Zhang, C., Helmsing, S., Zagrebelsky, M., Schirrmann, T., Marschall, A.L.J., Schüngel, M., Korte, M., Hust, M. and Dübel, S. (2012). Therapeutic Antibodies, Ed: Dimitrov, A.S.. (31) Thie, H., Voedisch, B., Dübel, S., Hust, M. and Schirrmann, T. (2009). and Reinard, T. (2002). Kai-Thomas Schneider adds: “We have all learned a lot and have grown together as a team. Technische Universitat (TU) Braunschweig is a public university situated in the Lower Saxony city of Braunschweig in Germany. Development of Germline-Humanized Antibodies Neutralizing Botulinum Neurotoxin A and B. September 2019, die Norden Vaccines GmbH gegründet. Utilisation of antibody microarrays for the selection of specific and informative antibodies from recombinant library binders of unknown quality. Bildnachweis: Michael Hust/TU Braunschweig. and Rhiel, E. (1999). (2017). (36) Hust, M., Steinwand, M., Al-Halabi, L., Helmsing, S., Schirrmann, T. and Dübel, S. (2009). (143) Moreira, G. M.S, Köllner, S.M.S., Helmsing, S., Jänsch, L., Meier, A., Gronow, S.,  Boedeker, C.,  Dübel, S,  Mendonça, M.,  Moreira, A. N., Conceição, F. R., and Hust, M. (in press). (100) Schieferdecker, A., Oberle, A., Thiele, B., Hofmann, F., Göthel, M, Miethe, S., Hust, M., Braig, F., Voigt, M., Koch-Nolte, F., Haag, F., Alawi, M., Indenbirken, D., Grundhoff, A., Bokemeyer, C., Bacher, U., Kröger, N. and Binder, M. (2016). “At first we don’t test with the real corona virus, but only with part of the virus’ surface. Application of M13 Phage Display for Identifying Immunogenic Proteins from Tick (Ixodes scapularis) Saliva. Karriere an der TU Braunschweig. and Hust, M.. (118) Avril, A., Miethe, S., Hust, M. and Pelat, T. (2018). (55) Hust, M., Meyer, T., Voedisch, B., Rülker, T., Thie, H., El-Ghezal, A., Kirsch, M.I., Schütte, M., Helmsing, S., Meier, D., Schirrmann, T. and Dübel, S. (2011). Vertical migration behaviour of diatom assemblages of Wadden Sea sediments (Dangast/Germany): A study using Cryo-scanning electron microscopy. (12) Konthur, Z., Hust, M. and Dübel, S. (2005). 38106 Braunschweig Norman Hack zusammen mit weiteren Instituten aus Braunschweig, München und Hannover Grundlagenforschung zum Thema „Additive Fertigung von Bauteilen“ betrieben. Phone: +49 531 391-5760 Michael Hust studierte von 1993 bis 1999 Biologie an der Carl von Ossietzky Universität Oldenburg . (49) Schirrmann, T. and Hust, M. (2010). (142) Dübel, S., Hust, M., Frenzel, A. Phage display-derived human antibodies in clinical development and therapy. (5)Department of Biochemistry and Biotechnology, Technical University Braunschweig, 38106 Braunschweig, Germany. Website: https://www.tu-braunschweig.de/bbt/biotech/corat-corona-antibody-team. Generation and analysis of the improved human HAL9/10 antibody phage display libraries. 38106 Braunschweig Ihr Ziel ist es, einen universellen Impfstoff gegen von Zecken übertragene Krankheiten zu entwickeln. (112) Schmitt, L.C., Rau, A., Seifert, O., Honer, J., Schmid, S., Hutt, M., Zantow, J., Hust, M., Dübel, S., Olayioye, M.A. Production of single chain Fab (scFab) in Bacillus megaterium. Production of recombinant antibody fragments in. C., Jordan, E., El-Ghezal, A., Schirrmann, T., Hust, M. und Jostock, T. (2005). Initiiert wurde das CORAT Konsortium von Professor Stefan Dübel und Professor Michael Hust vom Institut für Biochemie, Biotechnologie und Bioinformatik der TU Braunschweig. With this information, we can produce them again and again.”, This small tube contains an antibody library with about 10 billion antibody blueprints. (33) Hust, M., Thie, H., Schirrmann, T. and Dübel, S. (2009). (2017) The European AntibotABE Framework Program and Its Update: Development of Innovative Botulinum Antibodies. (80) Marschall, A.L.J., Zhang, C., Frenzel, A., Schirrmann, T., Hust, M., Perez, F. and Dübel, S (2014). Generating recombinant antibodies to the complete human proteome. Phage display, Ed: Lim, T.S. Selection of recombinant antibodies from antibody gene libraries. (148) König, J, Hust, M. and Van den Heuvel, J. Human Monoclonal Antibodies: Methods and Protocols, Ed: Steinitz, M.. (73) Kügler, J., Zantow, J., Meyer, T., and Hust, M. (2013). (34) Pelat, T., Hust, M., Hale, M., Lefranc, M.-P., Dübel, S. and Thullier, P. (2009). (81) Fuchs, M., Kämpfer, S., Helmsing, S., Spallek, R., Oehlmann, W., Prilop, W., Frank, R., Dübel, S., Mahavir, S. and Hust, M. (2014). (18) Schirrmann, T., Hust, M., Konthur, Z., Frank, R., Toleikis, L. and Dübel, S. (2007). Kontakt. Minimum Information about a Protein Affinity Reagent (MIAPAR). (10) Hust, M., Toleikis, L. and Dübel, S. (2005). Single chain antibodies as tools to study TGF-β regulated SMAD proteins in proximity ligation-based pharmacological screens. (89) Droste, P., Frenzel, A., Steinwand, M., Pelat, T., Thullier, P., Hust, M., Lashuel, H. and Dübel, S. (2015). Der Professor für Biotechnologie an der Technischen Universität Braunschweig ist selbst schon Opfer einer Zecke geworden, die ihn infizierte. Braunschweig TU Braunschweig ATAC (Antibody therapy against corona) Prof. Michael Hust TU Braunschweig, Karolinska Institut (Schweden), IRB (Schweiz), Joint Research Center Construction of Human Immune and Naive scFv Libraries. (2018). 5/2011 Habilitation (professorial dissertation, venia legendi for biotechnology), appointed as Privatdozent (PD). However, since SARS-CoV-2 is a novel virus, many people have not yet developed antibodies against it. Epitope Mapping via Phage Display from single gene libraries. (2016). (127) Froude, J.W., Herbert, A.S., Pelat, T., Miethe, S., Zak, S.E., Brannan, J.M., Bakken, R.R., Steiner, A.R., Yin, G., Hallam, T.J., Sato, A.K., Hust, M., Thullier, P. and Dye, J.M. barton.jessica@mh-hannover.de. Transient plant production of Salmonella Typhimurium diagnostic antibodies. A transplant immunome deep sequencing and screening platform defines a unique targetable epitope fingerprint of multiple myeloma. (123) Dübel, S., Hust, M., Kügler, J., Kuhn, P., Frenzel, A., Krah, S. and Zielonka, S. (2018). (72) Frenzel, A., Hust, M. and Schirrmann, T. (2013). Kai-Thomas Schneider and Nora Langreder arrived in a good mood and with mouth and nose protection for our conversation via video conference. A gene library of this kind comprises around 10 billion antibody blueprints. Development of neutralizing scFv-Fc against botulinum neurotoxin A light chain from a macaque immune library. (78) Hülseweh, B., Rülker, T., Pelat, T., Langermann, C., Frenzel, A., Schirrmann, T., Dübel, S., Thullier, P. and Hust, M. (2014). (96) Kibat, J., Schirrmann, T., Knape, M.J., Helmsing, S., Meier, D., Hust, M., Schröder, C., Bertinetti, D., Pardes, K., Funk, M., Lages, A., Giese, N., Herberg, F. W., Dübel, S. and Hoheisel, J.D. Generation of recombinant antibodies against toxins and viruses by phage display for Diagnostics and Therapy. (103) Frenzel, A., Schirrmann, T. and Hust, M. (2016). Improved microtitre plate production of single chain Fv fragments in Escherichia coli. Generation and characterization of protective antibodies to Marburg virus. “This is a method of producing antibodies in the test tube without the immunisation of animals,” explains Stefan Dübel, one of the inventors of the method. since 12/2002 Group leader at the Institut für Biochemie und Biotechnologie, Abteilung Biotechnologie, Technische Universität Braunschweig headed by Prof. Dr. Stefan Dübel. Picture credits: Michael Hust/TU Braunschweig. (132) Ramli, R.S., Moreira, G.M.S.G., Zantow, J., Goris, M.G.A., Nguyen, V.K., Novoselova, N., Pessler, F. and Hust, M. (2019). Supernatant was harvested after 48 h. That is our motivation.”. Discovery of Leptospira spp. The conserved Cys-2232 in Clostridioides difficile toxin B modulates oligomerization and receptor binding. (139) Wenzel, E.V., Bosnak, M., Tierney, R., Schubert, M., Brown, J., Dübel, S., Efstratiou, A., Sesardic, D., Sticking, P. and Hust, M. (2020). (13) Hust, M., Meysing, M, Schirrmann, T., Selke, M., Meens, J., Gerlach, G.-F. and Dübel, S. (2006). (101) Zantow, J., Dübel, S. and Hust, M. (2016). (130) Fühner, V., Heine, P.A., Helmsing, S., Goy, S., Heidepriem, J., Loeffler, F.F., Dübel, S., Gerhard, R. and Hust, M. (2018). The production of a genus specific recombinant antibody (scFv) using a recombinant Potyvirus protease. (99) Rohrbeck, A., Fühner, V., Schröder, A., Hagemann, S., Vu, X.-H., Berndt, S., Hust, M., Pich, A. and Just, I. (126) Burke, C.W., Froude, J.W., Miethe, S., Hülseweh, B., Hust, M. and Glass, P.J. Production of Single Chain Fragment Variable (scFv) Antibodies in Escherichia coli Using the LEX Bioreactor. The two research projects “QuantumFrontiers” and “SE²A” will be funded for seven years starting in 2019. (131) Fühner, V., Heine, P.A., Zilkens, K., Meier, D., Roth, K.D.R., Moreira, G.M.S.G., Hust, M. and Russo, G. (2019). Fuctional knockdown of VCAM-1 at the posttranslational level with ER retained antibodies. SRP and Sec pathway leader peptides for antibody phage display and antibody fragment production in E. coli. B., Hust, M. and Zielonka S. (in press). and Hust, M.. (116) Frenzel, A., Kügler, J., Helmsing, S., Meier, D., Schirrmann, T., Hust, M. and Dübel, S. (2017). First lab space was located in the GBF (National Research Centre for Biotechnology, Braunschweig, now: Helmholtz Centre for Infection Reserach). Braunschweiger Forscher gehen den Zecken an den Kragen Jüngste Ausgründung aus der TU Braunschweig Wissenschaftler aus Braunschweig haben heute, am 11. Therapeutic Antibodies, Ed: Dimitrov, A.S.. (30) Strebe, N., Guse, A., Schüngel, M., Schirrmann, T., Haffner, M., Jostock, T., Hust, M., Müller, W. and Dübel, S. (2009). ➜ Research projects: The Collaborative Research Centres, Research Groups and Priority Programmes funded by the German Research Foundation (DFG) play a special role. and Hust, M.. (120) Unkauf, T., Hust, M. and Frenzel, A. Department of Biotechnology Entwicklung von therapeutischen Antikörpern im Reagenzglas. m.hust@tu-braunschweig.de. Picture credits: Michael Hust/TU Braunschweig. Analysis of single chain antibody sequences using the VBASE2 Fab Analysis Tool. The consortium scientists are confident that they will also be able to find a suitable candidate against the corona virus. (71) Jäger, V.,  Büssow, K., Wagner, A., Weber, S., Hust, M., Frenzel, A. and Schirrmann, T. (2013). Identification of Novel Immunogenic Proteins of Neisseria gonorrhoeae by Phage Display. (67) Al-Halabi, L., Balck, A., Michalzik, M., Fröde, D., Büttgenbach, S., Hust, M., Schirrmann, T. and Dübel, S. (2013). and Kontermann, R.E. Mining gut microbiome oligopeptides by functional metaproteome display. Mit dieser Technologie können menschliche Antikörper im Reagenzglas generiert werden. (7) Schirrmann, T., Hust, M. und Dübel, S. (2005). Die TU Braunschweig (Prof. Michael Hust) ist Teil des COVID19-Konsortiums "ATAC" im Rahmen des EU Horizon2020 Corona-Förderprogramms. Die Daten dienen dazu, das Webangebot zu optimieren.Weitere Informationen finden Sie in unserer Datenschutzerklärung. In: Antibody engineering Vol 2, Ed: Kontermann, R. and Dübel, S.. (45) Mollova, S., Retter, I., Hust, M., Dübel, S. and Müller, W. (2010). Antibody Phage Display. (87) Kügler, J., Wilke, S. Meier, D., Tomszak, F.,  Frenzel, A. , Schirrmann, T., Dübel, S.,  Garritsen, H.,  Hock, B., Toleikis, L., Schütte, M. and Hust, M. (2015). (144) Laureano, A.F.S., Zani, M.B., Sant'Ana, A.M., Tognato, R.C., Lombello, C.B., do Nascimento, M.H.M., Helmsing, S., Fühner, V., Hust, M. and Puzer, L (2020). (17) Hust, M., Jostock, T., Menzel, C., Voedisch, B., Mohr, A., Brenneis, M., Kirsch, M.I., Meier, D. and Dübel, S. (2007). Once our project was designed, we sought advice from specialists in the field. (108) Postel, S., Deredge, D., Bonsor, D.A., Yu, X., Diederichs, K., Helmsing, S., Vromen, A., Friedler, A., Hust, M., Egelman, E.H., Beckett, D., Wintrode, P.L. Restriction-Free Construction of a Phage-Presented Very Short Macrocyclic Peptide Library. www.bbt.tu-bs.de/Biotech, Technische Universität Braunschweig A high affinity, human like antibody fragment (scFv) neutralising lethal factor (LF) of Bacillus anthracis by inhibiting the PA-LF complex formation. In addition, the team has also created such libraries from the blood of cured corona patients because the probability of finding a suitable antibody is even greater in such cases. Prof. Dr.-Ing. Antibody production in Bacillus megaterium: strategies and physiological implications of scaling from micro titer plates to industrial bioreactors. Michael Hust weiß, wovon er redet, wenn er über die Borreliose spricht. Expression rekombinanter Proteinpharmazeutika. (15) Soltes, G., Hust, M., Ng, K.K.Y., Bansal, A., Field, J., Stewart, D.I.H., Dübel, S., Cha, S. and Wiersma, E. (2007). (95) Tomszak, F., Weber, S., Zantow, J., Schirrmann, T., Hust, M. and Frenzel, A. Recombinant VHHA6 cDNA was cloned into the pCMX expression vector (contributed by M. Hust, TU Braunschweig), which encodes an optimized human IgG1 Fc domain. and Rhiel, E. (2003). (107) Zantow, J., Just, S., Lagkouvardos, I., Kisling, S., Dübel, S., Lepage, P., Clavel, T. and Hust, M. (2016). (137) Jakob, V., Helmsing, S., Hust, M. and Empting, M. (2020). (16) Jordan, E., Hust, M., Roth, A., Biedendieck, R., Schirrmann, T., Jahn, D. and Dübel, S. (2007). (69) Miethe, S., Meyer, T., Wöhl-Bruhn, S.,  Frenzel. Email: s.duebel@tu-braunschweig.de (146) Bertoglio, F., Fühner. Generation of recombinant antibodies for human tissue kallikrein 7 to address skin diseases. Novel human recombinant antibodies against Mycobacterium tuberculosis antigen 85B. (2018). Structural differences of amyloid-β fibrils revealed by antibodies from phage display. Michael Hust | Metropolregion Hannover-Braunschweig-Göttingen-Wolfsburg | Professor für Biotechnologie bei Technische Universität Braunschweig | 415 Kontakte | Vollständiges Profil von Michael auf LinkedIn anzeigen und vernetzen Ausbildung und Beruf. Expression of recombinant Antibodies. Normally, the corresponding approval procedure takes about one and a half years or more. and Hust, M.. (117) Kügler, J., Tomszak, F., Frenzel, A. and Hust, M. (2018). Braunschweig. 5/2011 Habilitation (professorial dissertation, venia legendi for biotechnology), appointed as Privatdozent (PD). A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients by phage display is binding to the ACE2-RBD interface and is tolerant to known RBD mutations. Human-Like Neutralizing Antibodies Protect Mice from Aerosol Exposure with Western Equine Encephalitis Virus. (26) Thie, H., Schirrmann, T., Paschke, M., Dübel, S. and Hust, M. (2008). whether the antibody is able to inhibit the corona virus from docking to the cells. Perspectives for systematic in vitro antibody generation. Kai-Thomas Schneider and Nora Langreder are PhD students at the Institute for Biochemistry, Biotechnology and Bioinformatics at the Technische Universität Braunschweig. The work now feels like a doctoral thesis on a much larger scale and in less time, which we are all doing together as a team. Isolation of a nanomolar scFv inhibiting the endopeptidase activity of botulinum toxin A, by single-round panning of an immune phage-displayed library of macaque origin. (54) Dammeyer, T., Steinwand, M., Krüger, S.C., Dübel, S., Hust, M. and Timmis, K.N. It works great because we can rely on each other,” says Nora Langreder describing the collaboration. (75) Steinwand, M., Droste, P., Frenzel A., Hust, M., Dübel, S. and Schirrmann, T. (2014). 1 Job ist im Profil von Wolfgang Niemeier aufgelistet. Post-Exposure Protection in Mice against Sudan Virus by a Two Antibody Cocktail. The binary toxin CDT of Clostridium difficile as tool for intracellular delivery of bacterial glucosyltransferase domains. ➜ Clusters of Excellence: In the Excellence Strategy of the Federal Government and States in 2018, TU Braunschweig was successful with two applications. The influence of antibody fragment format on phage display based affinity maturation of IgG. We are particularly grateful that the technical staff here also provides us with fantastic support. High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells. Identification of a putative Crf splice variant and generation of recombinant antibodies for the specific detection of Aspergillus fumigatus. Researchers at the Department of Biotechnology of Technische Universität Braunschweig started a research project to develop novel antibody based therapy for coronavirus infections. (2013). Targeting Aspergillus fumigatus Crf Transglycosylases With Neutralizing Antibody Is Relevant but Not Sufficient to Erase Fungal Burden in a Neutropenic Rat Model. (114) Stech, M., Nikolaeva, O., Thoring, L., Stöcklein, W.F.M., Wüstenhagen, D.A., Hust, M., Dübel, S. and Kubick, S. (2017). since 12/2002 Group leader at the Institut für Biochemie und Biotechnologie, Abteilung Biotechnologie, Technische Universität Braunschweig headed by Prof. Dr. Stefan Dübel. Construction of human antibody gene libraries and selection of antibodies by phage display. International Microbiology 2, 103-110, Stellen der TU Braunschweig Jobbörse des Career Service Merchandising Sponsoring- & Spendenleistungen Drittmittelgeförderte Forschungsprojekte, Semestertermine Lehrveranstaltungen Studiengänge von A-Z Informationen für Erstsemester TUCard, Status GITZ-Dienste Handbuch für TYPO3 (Intern) Corporate Design-Toolbox (Intern) Glossar (DE-EN) Meine Daten ändern Hochschulöffentliche Bekanntmachungen, Technische Universität Braunschweig Universitätsplatz 2 38106 Braunschweig Postfach: 38092 Braunschweig Telefon: +49 (0) 531 391-0. Wir sind auch dankbar für die rasche Anschubfinanzierung aus dem Land Niedersachsen, und die Förderung durch die Deutsche Herzstiftung e.V., Frankfurt a.M. So-called antibody gene libraries contribute to this. Phage display, Ed: Lim, T.S. In 1980, the Institute partly moved to the main campus at the TU Braunschweig. Lecturer for molecular biotechnology. Human-like antibodies neutralizing Western equine encephalitis virus. and Sundberg, E.J. They are part of the CORAT consortium that is developing a passive immunotherapy against the corona virus. (22) Schirrmann, T., Hust, M. and Dübel, S. (2007). © Copyright 2015 - 2020 TU Braunschweig | Blogs, Insights into the work of the Corona Antibody Team, Institute of Biochemistry, Biotechnology and Bioinformatics, Institute for Biochemistry, Biotechnology and Bioinformatics, Collaborative Research Centres, Research Groups and Priority Programmes. P., Sesardic, D. and Hust, M. (2015). Journal of Microbiological Methods 38, 69-80, (1) Janssen, M., Hust, M., Rhiel, E. and Krumbein, W.E. (93) Rasetti-Escargueil, C., Avril, A., Tierney, R., Bak, N., Miethe, S., Chahboun, S., Mazuet, C., Popoff, M., Thullier, P., Hust, M., Pelat, T. and Sesardic, D. (2015). Phage display derived therapeutic antibodies. Nature Methods 8, 551-558, (6) Hust, M. and Dübel, S. (2005). Pflegedienst Pflege to Hus Herzlich Willkommen. (136) Tenspolde, M., Zimmermann, K., Weber, L.C., Hapke, M., Lieber, M., Dywicki, J., Frenzel, A., Hust, M., Galla, M., Buitrago-Molina, L.E., Manns, M.P., Jaeckel, E. and Hardtke-Wolenski, M. (2019). Parallelized antibody selection in microtiter plates. (141) Tundidor, Y., Ponce, L., Chao, L., Solozabal, J., Hust, M., Dübel, S. & Rojas, G. (2020). Institute for Biochemie, Biotechnologie und Bioinformatik Cell-free synthesis of functional antibodies using a coupled in vitro transcription-translation system based on CHO cell lysates. Phage display for the generation of antibodies for proteome research, diagnostics and therapy. The two PhD students Nora Langreder and Kai-Thomas Schneider work on the CORAT consortium. E.,  Condemine, F., Bottex, C., Vidal, D., Lefranc, M.P., Dübel, S. and Thullier, P. (2007). (140) Sellmann, C., Pekar, L., Bauer, C., Ciesielski, E., Krah, S., Becker, S., Toleikis, L., Kügler, J., Frenzel, A., Valldorf,. Passive immunotherapy, which is also being researched by the CORAT consortium, could help here: the administered antibodies could block the pathogen and thus provide protection until the immune system has produced its own antibodies. Stefan Dübel/TU Braunschweig; HZI/Verena Meier; Hallbauer_Fioretti; Michael Hust/TU Braunschweig; Kai Mattern/Institut für Mikrotechnik/TU Braunschweig; PLRI; Foto: Falk-Martin Drescher / Braunschweig Stadtmarketing GmbH (37) Thie, H., Binius, S., Schirrmann, T., Hust, M. and Dübel, S. (2009). They were obtained from human blood in pre-Corona times and, put simply, contain the information for the basic construction of practically all antibodies that the human species can produce,” explains Kai-Thomas Schneider. and Aleixo, J.A.. (2016). (98) Blokzijl, A., Zieba, A., Hust, M., Schirrmann, T., Helmsing, S., Grannas, K., Hertz, E., Moren, A., Chen, L., Soderberg, O., Moustakas, A., Dübel, S. and Landegren, U.